diff --git a/AnalysisTool-release/bin/log/20250618.log b/AnalysisTool-release/bin/log/20250618.log
index 2b1b55f..ef16774 100644
--- a/AnalysisTool-release/bin/log/20250618.log
+++ b/AnalysisTool-release/bin/log/20250618.log
@@ -36,3 +36,90 @@
 [2025-06-18 10:04:14,239] Selected Curve:new.xlsx
 [2025-06-18 10:04:17,332] xMax:94.8474
 [2025-06-18 10:04:25,159] Selected Curve:new.xlsx
+[2025-06-18 14:20:14,656] main...
+[2025-06-18 14:20:14,656] config file existed. 
+[2025-06-18 14:20:14,667] version:1.0.4.0
+[2025-06-18 14:20:14,676] setEventHandlerEnable...0
+[2025-06-18 14:20:53,900] slotSendData:9
+[2025-06-18 14:20:53,900] slotSendData:a5 5a 06 83 50 00 4a a8 46
+[2025-06-18 14:20:53,900] All data writen.
+[2025-06-18 14:20:53,901] open serial port.
+[2025-06-18 14:25:16,037] main...
+[2025-06-18 14:25:16,039] config file existed. 
+[2025-06-18 14:25:16,049] version:1.0.4.0
+[2025-06-18 14:25:16,059] setEventHandlerEnable...0
+[2025-06-18 14:25:28,907] main...
+[2025-06-18 14:25:28,907] config file existed. 
+[2025-06-18 14:25:28,918] version:1.0.4.0
+[2025-06-18 14:25:28,927] setEventHandlerEnable...0
+[2025-06-18 14:25:55,875] slotSendData:9
+[2025-06-18 14:25:55,875] slotSendData:a5 5a 06 83 50 00 4a a8 46
+[2025-06-18 14:25:55,875] All data writen.
+[2025-06-18 14:25:55,876] open serial port.
+[2025-06-18 14:26:36,472] phase 2 enable.
+[2025-06-18 14:26:36,472] slotSendData:82
+[2025-06-18 14:26:36,472] slotSendData:a5 5a 4f 82 50 00 01 00 00 48 43 00 00 a0 41 05 00 01 01 00 00 48 43 00 00 80 3f 3c 00 01 00 00 00 00 00 00 00 00 00 00 00 01 00 00 00 00 00 00 00 00 00 00 00 01 00 00 00 00 00 00 00 00 00 00 00 01 00 00 00 00 00 00 00 00 00 00 00 01 00 01 a2 34
+[2025-06-18 14:26:36,472] All data writen.
+[2025-06-18 14:29:32,803] phase 2 enable.
+[2025-06-18 14:29:32,803] slotSendData:82
+[2025-06-18 14:29:32,803] slotSendData:a5 5a 4f 82 50 00 01 00 00 48 43 00 00 a0 41 05 00 01 01 00 00 48 43 00 00 80 3f 3d 00 01 00 00 00 00 00 00 00 00 00 00 00 01 00 00 00 00 00 00 00 00 00 00 00 01 00 00 00 00 00 00 00 00 00 00 00 01 00 00 00 00 00 00 00 00 00 00 00 01 00 01 3a 91
+[2025-06-18 14:29:32,803] All data writen.
+[2025-06-18 14:29:47,987] start experiment,set soft into experiment mode.
+[2025-06-18 14:29:47,987] clearExperimentData...
+[2025-06-18 14:29:47,990] slotSendData:9
+[2025-06-18 14:29:47,990] slotSendData:a5 5a 06 82 2c 00 01 28 55
+[2025-06-18 14:29:47,990] All data writen.
+[2025-06-18 14:31:02,677]  Stop experiment ...
+[2025-06-18 14:31:02,677] slotSendData:9
+[2025-06-18 14:31:02,677] slotSendData:a5 5a 06 82 2c 00 00 e9 95
+[2025-06-18 14:31:02,677] All data writen.
+[2025-06-18 14:31:05,153] filePath:D:/gitfile/analysis_tool/AnalysisTool-release/experiment_data/sample_data/氧化诱导期33.xlsx
+[2025-06-18 14:31:05,153] writeFile...
+[2025-06-18 14:31:05,153] write file...
+[2025-06-18 14:31:05,154] phase vtr size:0
+[2025-06-18 14:31:05,154] before xlsx save as...
+[2025-06-18 14:31:15,551] xlsx sample weight:7
+[2025-06-18 14:31:15,551] Cell does not exist.
+[2025-06-18 14:31:17,743] xlsx sample weight:1
+[2025-06-18 14:31:17,743] startLineIndex:11
+[2025-06-18 14:31:17,743] data size:298
+[2025-06-18 14:31:17,743] Cell does not exist.
+[2025-06-18 14:31:19,256] xlsx sample weight:0
+[2025-06-18 14:31:19,256] startLineIndex:11
+[2025-06-18 14:31:19,256] data size:3550
+[2025-06-18 14:31:19,259] Cell does not exist.
+[2025-06-18 14:31:20,971] clearExperimentData...
+[2025-06-18 14:31:21,999] xlsx sample weight:7
+[2025-06-18 14:31:21,999] Cell does not exist.
+[2025-06-18 14:39:44,122] start experiment,set soft into experiment mode.
+[2025-06-18 14:39:44,122] clearExperimentData...
+[2025-06-18 14:39:44,125] slotSendData:9
+[2025-06-18 14:39:44,125] slotSendData:a5 5a 06 82 2c 00 01 28 55
+[2025-06-18 14:39:44,125] All data writen.
+[2025-06-18 14:39:56,333]  Stop experiment ...
+[2025-06-18 14:39:56,333] slotSendData:9
+[2025-06-18 14:39:56,333] slotSendData:a5 5a 06 82 2c 00 00 e9 95
+[2025-06-18 14:39:56,333] All data writen.
+[2025-06-18 14:40:02,447] filePath:D:/gitfile/analysis_tool/AnalysisTool-release/experiment_data/sample_data/氧化诱导期33.xlsx
+[2025-06-18 14:40:02,447] writeFile...
+[2025-06-18 14:40:02,447] write file...
+[2025-06-18 14:40:02,447] phase vtr size:0
+[2025-06-18 14:40:02,447] before xlsx save as...
+[2025-06-18 14:40:16,204] phase 2 enable.
+[2025-06-18 14:40:16,204] slotSendData:82
+[2025-06-18 14:40:16,204] slotSendData:a5 5a 4f 82 50 00 01 00 00 48 43 00 00 a0 41 05 00 01 01 00 00 48 43 00 00 80 3f 3d 00 01 00 00 00 00 00 00 00 00 00 00 00 01 00 00 00 00 00 00 00 00 00 00 00 01 00 00 00 00 00 00 00 00 00 00 00 01 00 00 00 00 00 00 00 00 00 00 00 01 00 01 3a 91
+[2025-06-18 14:40:16,204] All data writen.
+[2025-06-18 14:40:20,620] start experiment,set soft into experiment mode.
+[2025-06-18 14:40:20,620] clearExperimentData...
+[2025-06-18 14:40:20,625] slotSendData:9
+[2025-06-18 14:40:20,625] slotSendData:a5 5a 06 82 2c 00 01 28 55
+[2025-06-18 14:40:20,625] All data writen.
+[2025-06-18 14:40:47,212]  Stop experiment ...
+[2025-06-18 14:40:47,212] slotSendData:9
+[2025-06-18 14:40:47,212] slotSendData:a5 5a 06 82 2c 00 00 e9 95
+[2025-06-18 14:40:47,212] All data writen.
+[2025-06-18 14:40:50,598] filePath:D:/gitfile/analysis_tool/AnalysisTool-release/experiment_data/sample_data/氧化诱导期33.xlsx
+[2025-06-18 14:40:50,598] writeFile...
+[2025-06-18 14:40:50,598] write file...
+[2025-06-18 14:40:50,598] phase vtr size:0
+[2025-06-18 14:40:50,598] before xlsx save as...
diff --git a/AnalysisTool-release/experiment_data/sample_data/氧化诱导期33.xlsx b/AnalysisTool-release/experiment_data/sample_data/氧化诱导期33.xlsx
new file mode 100644
index 0000000..28cc029
Binary files /dev/null and b/AnalysisTool-release/experiment_data/sample_data/氧化诱导期33.xlsx differ
diff --git a/experiment_data/sample_data/new.xlsx b/experiment_data/sample_data/new.xlsx
index 3685f14..db680cd 100644
Binary files a/experiment_data/sample_data/new.xlsx and b/experiment_data/sample_data/new.xlsx differ
diff --git a/src/data/xlsxhandler.cpp b/src/data/xlsxhandler.cpp
index 3a62cfb..3ba6576 100644
--- a/src/data/xlsxhandler.cpp
+++ b/src/data/xlsxhandler.cpp
@@ -251,6 +251,10 @@ void XlsxHandler::writeExperimentFile(const QString filePath)
         row++;
 
         // phase data.
+        if(Global::_curveExperimentDataVtr.size() < i + 1){
+            continue;
+        }
+
         QVector<Global::ExperimentData> edVtr =
                 Global::_curveExperimentDataVtr.at(i).dataVtr;
 
diff --git a/src/global.cpp b/src/global.cpp
index e4ff3f7..bb7b2d5 100644
--- a/src/global.cpp
+++ b/src/global.cpp
@@ -26,6 +26,8 @@ bool _displayTimeValue = false;
 
 LanguageType _languageType = LanguageType::Chinese;
 
+bool _experimentOITFlag = false;
+
 QString converDoubleToStr(const double num)
 {
     return QString::number(num,'f',3);
diff --git a/src/global.h b/src/global.h
index 9789697..dd3a5b3 100644
--- a/src/global.h
+++ b/src/global.h
@@ -158,6 +158,8 @@ extern LanguageType _languageType;
 
 extern AxisMode _axisMode;
 
+extern bool _experimentOITFlag;
+
 // common func
 QString converDoubleToStr(const double);
 void quadraticLeastSquaresFit(double x[], double y[], int n, double coeff[]);
diff --git a/src/mainwindow.cpp b/src/mainwindow.cpp
index cafcf6b..28b41b3 100644
--- a/src/mainwindow.cpp
+++ b/src/mainwindow.cpp
@@ -478,6 +478,8 @@ void MainWindow::on_actionStart_triggered()
     Global::_mode = Global::Mode::Experiment;
 
     _manuallyStopTheExperimentFlag = false;
+
+    _centralWidget->startExperiment();
 }
 
 void MainWindow::on_actionReadOnly_triggered()
diff --git a/src/serialport/serialport.h b/src/serialport/serialport.h
index 45612e6..aa02476 100644
--- a/src/serialport/serialport.h
+++ b/src/serialport/serialport.h
@@ -22,8 +22,7 @@ public:
     static SerialPort *instance();
     ~SerialPort();
 
-    void sendData(const QByteArray &data)
-    {   slotSendData(data);    }
+    void sendData(const QByteArray &data){slotSendData(data);}
     void sendCmd(const E_CMD_TYPE e);
     static void parserTest();
     bool openSp();
diff --git a/src/ui/centralwidget.cpp b/src/ui/centralwidget.cpp
index 1b9cd4f..8f618c6 100644
--- a/src/ui/centralwidget.cpp
+++ b/src/ui/centralwidget.cpp
@@ -197,6 +197,22 @@ QCPCurve* CentralWidget::addCurveData(
     return _currentCurve;
 }
 
+void CentralWidget::startExperiment()
+{
+    if(Global::_experimentOITFlag){
+        setAxisMode(Global::AxisMode::DoubleY);
+    }else{
+        setAxisMode(Global::AxisMode::SingleY);
+    }
+
+    if(_yAxis2Curve != nullptr){
+        _yAxis2Curve = nullptr;
+    }
+    _customPlot->yAxis2->setRange(0, 400);
+    _customPlot->xAxis->setRange(-5, 400);
+    _customPlot->yAxis->setRange(-20, 20);
+}
+
 void CentralWidget::setAnalysisMode(const AnalysisMode mode)
 {
     _analysisMode = mode;
@@ -260,19 +276,7 @@ void CentralWidget::slotModeModify(const Global::Mode mode)
 
 void CentralWidget::slotRecvCommonData(const CommonData &cd)
 {
-    static double index = 0.0;
-
-    if(!_currentCurve){
-        logde<<"_currentCurve is nullptr";
-        _currentCurve = new QCPCurve(_customPlot->xAxis, _customPlot->yAxis);
-        _currentCurve->setObjectName(Global::ObjectNameExperiemnt);
-    }
-
-    //    logde<<"temp:"<<cd.sample_temp<<",dsc:"<<cd.dsc;
-    _currentCurve->addData(index++,cd.sample_temp, cd.dsc);
-
-    // _customPlot->rescaleAxes();
-    _customPlot->replot();
+    logde<<"slotRecvCommonData run time:"<<cd.add_run_time;
 
     // Record data.
     if(!Global::_currentCurveExperimentDataPtr){
@@ -287,12 +291,60 @@ void CentralWidget::slotRecvCommonData(const CommonData &cd)
 
         if(Global::_currentCurveExperimentDataPtr->dataVtr.empty()){
             ed.phaseIndex = Global::_currentCurveExperimentDataPtr->phaseIndex;
+            logde<<"current phase index:"<<Global::_currentCurveExperimentDataPtr->phaseIndex;
+            _currentCurve = nullptr;
         }
 
         Global::_currentCurveExperimentDataPtr->dataVtr.push_back(ed);
+    }
+
+    // Update ui.
+    static double index = 0.0;
+
+    if(!_currentCurve){
+        logde<<"_currentCurve is nullptr";
+        _currentCurve = new QCPCurve(_customPlot->xAxis, _customPlot->yAxis);
+        _currentCurve->setObjectName(Global::ObjectNameExperiemnt);
+
         Global::_currentCurveExperimentDataPtr->curve = _currentCurve;
     }
 
+    if(!Global::_experimentOITFlag){
+        _currentCurve->addData(index++,cd.sample_temp, cd.dsc);
+    }else{
+        if(_yAxis2Curve == nullptr){
+            _yAxis2Curve = new QCPCurve(_customPlot->xAxis, _customPlot->yAxis2);
+            _yAxis2Curve->setSelectable(QCP::stWhole);     // 设置曲线可选
+            _yAxis2Curve->setLineStyle(QCPCurve::lsLine);  // 线性连接
+
+            _yAxis2Curve->setObjectName(Global::ObjectNameExperiemnt);
+
+            // 设置曲线颜色
+            QPen pen(Qt::red); // 创建一个红色的画笔
+            pen.setWidth(1); // 设置画笔宽度
+            _yAxis2Curve->setPen(pen); // 将画笔应用到曲线
+        }
+        _yAxis2Curve->addData(index++,cd.add_run_time, cd.sample_temp);
+
+        _currentCurve->addData(index++,cd.add_run_time, cd.dsc);
+
+        //    _customPlot->yAxis2->setRange(0, 400);
+    }
+
+    // Set asis xy range.
+    // axis dsc -20 20
+    // time 0 100
+    // temp 0 400
+
+    //    _customPlot->xAxis->setRange(0, 400);
+    //    _customPlot->yAxis->setRange(-20, 20);
+
+
+    // _customPlot->rescaleAxes();
+    _customPlot->replot();
+
+
+
 #if 0
     logde<<"_curveExperimentDataVtr size:"
         <<Global::_curveExperimentDataVtr.size();
@@ -583,6 +635,7 @@ void CentralWidget::slotSelectionChangedByUser()
 
 void CentralWidget::uiLoadXlsxFileData()
 {
+#if 0
     // Set axis.
     _customPlot->yAxis->setVisible(true);
     _customPlot->yAxis->setLabel(AxisDSC);
@@ -597,8 +650,12 @@ void CentralWidget::uiLoadXlsxFileData()
         _customPlot->yAxis2->setVisible(true);
         _customPlot->yAxis2->setLabel(AxisTemperature);
     }
+#endif
 
 #if 1
+    if(Global::_curveFileDataVtr.empty()){
+        return;
+    }
     // Load xlsx file data.
     QVector<ExperimentData> allDataVtr;
     for(Global::CurveFileData& cfd:Global::_curveFileDataVtr){
@@ -608,26 +665,48 @@ void CentralWidget::uiLoadXlsxFileData()
             allDataVtr.append(pti.dataVtr);
 
             // Load data.
-            QVector<double> tVtr,xVtr, yVtr;
+            QVector<double> tVtr,tempVtr,timeVtr,dscVtr;
             int index = 0;
             for (Global::ExperimentData &ed : pti.dataVtr)
             {
                 tVtr.push_back(index++);
+#if 0
                 if(_axisMode == AxisMode::SingleY){
-                    xVtr.push_back(ed.sampleTemp);
+                    xTempVtr.push_back(ed.sampleTemp);
                 }else{
-                    xVtr.push_back(ed.runTime);
+                    xTimeVtr.push_back(ed.runTime);
                 }
-                yVtr.push_back(ed.dsc);
+#endif
+                tempVtr.push_back(ed.sampleTemp);
+                timeVtr.push_back(ed.runTime);
+
+                dscVtr.push_back(ed.dsc);
             }
 
             _currentCurve = new QCPCurve(_customPlot->xAxis, _customPlot->yAxis);
-            _currentCurve->setData(tVtr, xVtr, yVtr);
+            if(_axisMode == AxisMode::SingleY){
+                _currentCurve->setData(tVtr, tempVtr, dscVtr);
+            }else{
+                _currentCurve->setData(tVtr, timeVtr, dscVtr);
+            }
             _currentCurve->setSelectable(QCP::stWhole);     // 设置曲线可选
             _currentCurve->setLineStyle(QCPCurve::lsLine);  // 线性连接
-
             _currentCurve->setObjectName(cfd.filePath);
 
+            // add the second axis y curve data.
+            if(_axisMode == AxisMode::DoubleY){
+                QCPCurve *secondCurve = new QCPCurve(_customPlot->xAxis, _customPlot->yAxis2);
+                secondCurve->setData(tVtr, timeVtr, tempVtr);
+                secondCurve->setSelectable(QCP::stWhole);     // 设置曲线可选
+                secondCurve->setLineStyle(QCPCurve::lsLine);  // 线性连接
+
+                secondCurve->setObjectName(cfd.filePath);
+
+                // 设置曲线颜色
+                QPen pen(Qt::red); // 创建一个红色的画笔
+                pen.setWidth(1); // 设置画笔宽度
+                secondCurve->setPen(pen); // 将画笔应用到曲线
+            }
 #if 0
             // Set axis range.
             QPair<float, float> minMaxXAxisPair;
@@ -662,6 +741,13 @@ void CentralWidget::uiLoadXlsxFileData()
         }else{
             // Axis x is time value.
             minMaxXAxisPair = PointCalculate::getMinAndMaxOfRunTime();
+
+            // Set yAxis2 range.
+            QPair<float, float> minAndMaxPair = PointCalculate::getMinAndMaxOfSampleTemp();
+            QPair<float, float>y2AxisPair = PointCalculate::getMinAndMaxOfAxis(
+                        minAndMaxPair.first,minAndMaxPair.second);
+
+            _customPlot->yAxis2->setRange(y2AxisPair.first,y2AxisPair.second);
         }
         QPair<float, float>newXAxisPair = PointCalculate::getMinAndMaxOfAxis(
                     minMaxXAxisPair.first,minMaxXAxisPair.second);
@@ -689,15 +775,6 @@ void CentralWidget::uiLoadXlsxFileData()
 #endif
 }
 
-#if 0
-void CentralWidget::contextMenuEvent(QContextMenuEvent *event)
-{
-    qDebug()<<"left menu...";
-    QPoint point =  event->globalPos();
-    emit sigContextMenuShow(point);
-}
-#endif
-
 void CentralWidget::glassTransitionHandle(const double x1,const double x2,const QString objectName)
 {
     QPointF point1 = PointCalculate::getClosestPointByX(x1);
@@ -1502,7 +1579,6 @@ void CentralWidget::drawOITLine(
     logde<<"start/end offset:"
         <<startOffset<<","<<endOffset;
 
-
     double verticalLineStartY = startData.dsc - startOffset;
     double verticalLineEndY = startData.dsc - endOffset;
 
@@ -1556,6 +1632,24 @@ void CentralWidget::setAxisMode(AxisMode mode)
 {
     if(_axisMode != mode){
         _axisMode = mode;
+
+        // Set axis.
+        _customPlot->yAxis->setVisible(true);
+        _customPlot->yAxis->setLabel(AxisDSC);
+
+        if(_axisMode == AxisMode::SingleY){
+            _customPlot->xAxis->setLabel(AxisTemperature);
+
+            _customPlot->yAxis2->setVisible(false);
+        }else{
+            _customPlot->xAxis->setLabel(AxisTime);
+
+            _customPlot->yAxis2->setVisible(true);
+            _customPlot->yAxis2->setLabel(AxisTemperature);
+        }
+
+        _customPlot->replot();
+
         uiLoadXlsxFileData();
     }
 }
@@ -1575,6 +1669,8 @@ QPixmap CentralWidget::getPixMap()
 
 void CentralWidget::slotAxisModify(const float temp)
 {
+    logde<<"slotAxisModify...";
+
     _customPlot->setInteractions(QCP::iRangeDrag | QCP::iRangeZoom | QCP::iSelectPlottables);
 
     float value = temp + 20;
diff --git a/src/ui/centralwidget.h b/src/ui/centralwidget.h
index 3d55c42..eb581d9 100644
--- a/src/ui/centralwidget.h
+++ b/src/ui/centralwidget.h
@@ -51,6 +51,8 @@ public:
     QCPCurve* addCurveData(const QVector<Global::ExperimentData> &,const QString objectName);
 
     QCPCurve * getCurrentCurve(){return _currentCurve;}
+
+    void startExperiment();
 signals:
     void sigContextMenuShow(const QPoint);
     void sigSendLineXCoord(const int,const double);
@@ -122,7 +124,7 @@ private:
 private:
     AnalysisOperationRecorder::AnalysisMode _analysisMode;
     LocalCustomPlot *_customPlot;
-    QCPCurve * _currentCurve;
+    QCPCurve * _currentCurve,*_yAxis2Curve;
     EventHandler* _eventHandler;
     QCPItemStraightLine *_line1,*_line2;
     QVector<QCPItemStraightLine*> _lineVtr;
diff --git a/src/ui/experimentsettingform.cpp b/src/ui/experimentsettingform.cpp
index bfdeaa8..8af35be 100644
--- a/src/ui/experimentsettingform.cpp
+++ b/src/ui/experimentsettingform.cpp
@@ -212,6 +212,36 @@ void ExperimentSettingForm::uiSetPhaseEnable(const int index)
     }
 }
 
+GasType ExperimentSettingForm::conver2GasType(const int index)
+{
+    switch (index) {
+    case 0: return GasType::NC; break;    // 假设 GAS_AIR 是 gas_type 的枚举值
+    case 1: return GasType::N2; break;
+    case 2: return GasType::O2; break;
+    default: return GasType::NC; break; // 处理未知情况
+    }
+    return GasType::NC;
+}
+
+bool ExperimentSettingForm::phaseCufoffTempAndTempFlowCheck(const int phaseIndex,
+                                                            const float prePhaseCufoffTemp,
+                                                            const float currentPhaseCufoffTemp,
+                                                            const float tempFlow)
+{
+    if(tempFlow < 0){
+        phaseScanRateErrorMesgBox(phaseIndex);
+        return false;
+    }
+
+    if(prePhaseCufoffTemp != currentPhaseCufoffTemp
+            && tempFlow == 0){
+        phaseScanRateErrorMesgBox(phaseIndex);
+        return false;
+    }
+
+    return true;
+}
+
 void ExperimentSettingForm::slotPhase2StateChanged(int state)
 {
     qDebug() << "slotPhase2StateChanged:" << state;
@@ -379,8 +409,8 @@ void ExperimentSettingForm::on_pushButton_deliverData_clicked()
         phase.cutoff_temp = ui->phase_1_cutoff_temp->text().toFloat();
         phase.temp_flow = ui->phase_1_scan_rate->text().toFloat();
         phase.constant_temp_time_min = (uint16_t)ui->phase_1_constant_temp->text().toInt();
-        //        phase.gas = ui->comboBox_phase_1_atmosphere->currentIndex();
-        phase.gas = GasType::N2;
+
+        phase.gas = conver2GasType(ui->comboBox_phase_1_atmosphere->currentIndex());
 
         phaseVtr.push_back(phase);
         //
@@ -390,6 +420,7 @@ void ExperimentSettingForm::on_pushButton_deliverData_clicked()
             phaseScanRateErrorMesgBox(1);
             return;
         }
+
     }
 
     {
@@ -398,8 +429,7 @@ void ExperimentSettingForm::on_pushButton_deliverData_clicked()
         phase.cutoff_temp = ui->phase_2_cutoff_temp->text().toFloat();
         phase.temp_flow = ui->phase_2_scan_rate->text().toFloat();
         phase.constant_temp_time_min = (uint16_t)ui->phase_2_constant_temp->text().toInt();
-        //        phase.gas = ui->comboBox_phase_1_atmosphere->currentIndex();
-        phase.gas = GasType::N2;
+        phase.gas = conver2GasType(ui->comboBox_phase_2_atmosphere->currentIndex());
 
         phaseVtr.push_back(phase);
         //
@@ -411,10 +441,26 @@ void ExperimentSettingForm::on_pushButton_deliverData_clicked()
                 return;
             }
 
-            if(phase.temp_flow <= 0){
+            //
+#if 0
+            if(phase.temp_flow < 0){
                 phaseScanRateErrorMesgBox(2);
                 return;
             }
+
+            if(phase2CutoffTemp != phase1CutoffTemp
+                    && phase.temp_flow == 0){
+                phaseScanRateErrorMesgBox(2);
+                return;
+            }
+#endif
+            if(!phaseCufoffTempAndTempFlowCheck(2,
+                                                phase1CutoffTemp,
+                                                phase2CutoffTemp,
+                                                phase.temp_flow)){
+                return;
+            }
+
         }
     }
 
@@ -424,7 +470,7 @@ void ExperimentSettingForm::on_pushButton_deliverData_clicked()
         phase.cutoff_temp = ui->phase_3_cutoff_temp->text().toFloat();
         phase.temp_flow = ui->phase_3_scan_rate->text().toFloat();
         phase.constant_temp_time_min = (uint16_t)ui->phase_3_constant_temp->text().toInt();
-        phase.gas = GasType::N2;
+        phase.gas = conver2GasType(ui->comboBox_phase_3_atmosphere->currentIndex());
 
         phaseVtr.push_back(phase);
         //
@@ -435,11 +481,18 @@ void ExperimentSettingForm::on_pushButton_deliverData_clicked()
                 phaseCufoffTempErrorMesgBox(3);
                 return;
             }
-
+#if 0
             if(phase.temp_flow <= 0){
                 phaseScanRateErrorMesgBox(3);
                 return;
             }
+#endif
+            if(!phaseCufoffTempAndTempFlowCheck(3,
+                                                phase2CutoffTemp,
+                                                phase3CutoffTemp,
+                                                phase.temp_flow)){
+                return;
+            }
         }
     }
 
@@ -449,8 +502,8 @@ void ExperimentSettingForm::on_pushButton_deliverData_clicked()
         phase.cutoff_temp = ui->phase_4_cutoff_temp->text().toFloat();
         phase.temp_flow = ui->phase_4_scan_rate->text().toFloat();
         phase.constant_temp_time_min = (uint16_t)ui->phase_4_constant_temp->text().toInt();
-        //        phase.gas = ui->comboBox_phase_1_atmosphere->currentIndex();
-        phase.gas = GasType::N2;
+        phase.gas = conver2GasType(ui->comboBox_phase_4_atmosphere->currentIndex());
+
         phaseVtr.push_back(phase);
         //
         if(phase.onoff){
@@ -460,11 +513,18 @@ void ExperimentSettingForm::on_pushButton_deliverData_clicked()
                 phaseCufoffTempErrorMesgBox(4);
                 return;
             }
-
+#if 0
             if(phase.temp_flow <= 0){
                 phaseScanRateErrorMesgBox(4);
                 return;
             }
+#endif
+            if(!phaseCufoffTempAndTempFlowCheck(4,
+                                                phase3CutoffTemp,
+                                                phase4CutoffTemp,
+                                                phase.temp_flow)){
+                return;
+            }
         }
     }
 
@@ -474,8 +534,7 @@ void ExperimentSettingForm::on_pushButton_deliverData_clicked()
         phase.cutoff_temp = ui->phase_5_cutoff_temp->text().toFloat();
         phase.temp_flow = ui->phase_5_scan_rate->text().toFloat();
         phase.constant_temp_time_min = (uint16_t)ui->phase_5_constant_temp->text().toInt();
-        //        phase.gas = ui->comboBox_phase_1_atmosphere->currentIndex();
-        phase.gas = GasType::N2;
+        phase.gas = conver2GasType(ui->comboBox_phase_5_atmosphere->currentIndex());
 
         phaseVtr.push_back(phase);
         //
@@ -486,11 +545,18 @@ void ExperimentSettingForm::on_pushButton_deliverData_clicked()
                 phaseCufoffTempErrorMesgBox(5);
                 return;
             }
-
+#if 0
             if(phase.temp_flow <= 0){
                 phaseScanRateErrorMesgBox(5);
                 return;
             }
+#endif
+            if(!phaseCufoffTempAndTempFlowCheck(5,
+                                                phase4CutoffTemp,
+                                                phase5CutoffTemp,
+                                                phase.temp_flow)){
+                return;
+            }
         }
     }
 
@@ -500,8 +566,7 @@ void ExperimentSettingForm::on_pushButton_deliverData_clicked()
         phase.cutoff_temp = ui->phase_6_cutoff_temp->text().toFloat();
         phase.temp_flow = ui->phase_6_scan_rate->text().toFloat();
         phase.constant_temp_time_min = (uint16_t)ui->phase_6_constant_temp->text().toInt();
-        //        phase.gas = ui->comboBox_phase_1_atmosphere->currentIndex();
-        phase.gas = GasType::N2;
+        phase.gas = conver2GasType(ui->comboBox_phase_6_atmosphere->currentIndex());
 
         phaseVtr.push_back(phase);
         //
@@ -512,11 +577,18 @@ void ExperimentSettingForm::on_pushButton_deliverData_clicked()
                 phaseCufoffTempErrorMesgBox(6);
                 return;
             }
-
+#if 0
             if(phase.temp_flow <= 0){
                 phaseScanRateErrorMesgBox(6);
                 return;
             }
+#endif
+            if(!phaseCufoffTempAndTempFlowCheck(6,
+                                                phase5CutoffTemp,
+                                                phase6CutoffTemp,
+                                                phase.temp_flow)){
+                return;
+            }
         }
     }
     //
@@ -541,8 +613,16 @@ void ExperimentSettingForm::on_pushButton_deliverData_clicked()
     QByteArray initialBa;
     if(ui->radioButton_OIT->isChecked()){
         initialBa.append((char)0);
+
+        Global::_experimentOITFlag = true;
+
+        logde<<"Global::_experimentOITFlag = true;";
     }else{
         initialBa.append((char)1);
+
+        Global::_experimentOITFlag = false;
+
+        logde<<"Global::_experimentOITFlag = false;";
     }
 
     char index = ui->comboBox_initial_atmosphere->currentIndex();
@@ -805,6 +885,9 @@ void ExperimentSettingForm::slotRecvPhaseInfo(const QByteArray &ba)
     }else{
         ui->radioButton_OIT->setChecked(true);
         ui->radioButton_OIT_not->setChecked(false);
+        Global::_experimentOITFlag = true;
+
+        logde<<"Global::_experimentOITFlag = true;";
 #if 0
         QString oitNotRadioButtonName("radioButton_OIT_not");
         QRadioButton *oitNotRadioButton =
diff --git a/src/ui/experimentsettingform.h b/src/ui/experimentsettingform.h
index 2844315..ec292ac 100644
--- a/src/ui/experimentsettingform.h
+++ b/src/ui/experimentsettingform.h
@@ -31,6 +31,11 @@ private:
     //ui
     void uiReset();
     void uiSetPhaseEnable(const int index);
+    GasType conver2GasType(const int index);
+    bool phaseCufoffTempAndTempFlowCheck(const int phaseIndex,
+                                         const float prePhaseCufoffTemp,
+                                         const float currentPhaseCufoffTemp,
+                                         const float tempFlow);
     // slot
     void slotPhaseCheck();
     void slotPhase2StateChanged(int state);